Hello,
First lock all your folders and then go into setting of Espionage and there is a tab named Backup, check if the set destination is valid.
But even if it is, just select a new folder for as destination and then unlock and lock one of your small folders. The first time you lock the folder it should trigger a full backup of the folder. Allow it a minute or two, depending of the size of the folder, then check if there is a copy of your disk image in the new destination folder.
If all looks good and you do not get any errors, then unlock and lock each of the protected folders, one by one, each time allowing a minute or two for backup to complete.
Once all is done, you can delete the old backup destination folder.
let me know if it did not work.
Rgds
Zsolt